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Please use this identifier to cite or link to this item: https://dyhuir.dyhu.edu.tw/ir/handle/987654321/956

Title: 海藻中含硫醣脂之鑑定與純化
Identification and Purification of Sulfoglycolipids from Marine Macroalga (Porphyra crispate)
Authors: 蔡政融
Date: 2012
Issue Date: 2013-06-06T03:03:18Z
Abstract: 紫菜 (Porphyra crispata) 經冷凍乾燥及磨粉 (200 mesh) 後,以無水酒精萃取,紫菜酒精萃取物經 Diaion HP-20 樹脂分離後,得glycolipids,再以弱陰離子交換纖維素 (DEAE-cellulose, diethylaminoethyl-cellulose) 分離,經冰丙酮沉澱後,得 SQDG (sulfoquinovosyldiacylglycerol) 純品 ,收率為 0.032 % dry basis。紫菜之 SQDG 主要含 palmitic acid (C16:0, 33.3%)、EPA (C20:5n-3, 30.0%)、arachidonic acid (C20:4n-6, 12.7%)、oleic acid (C18:1n-9, 7.52%) 和 stearic acid (C18:0, 6.83%) 等脂肪酸,不飽和脂肪酸n - 3 / n - 6 比值為 1.9,其抑制人類肝癌腫瘤細胞 (Hep G2) 之半抑制濃度 (IC50 ) 為126 μg /mL,低於菠菜SQDG(255μg / mL)。
A red alga, Porphyra crispata, was extracted with ethanol and purified on a hydrophobic column (Diaion HP-20 column) to obtain sulfoglycolipids (sulfoquinovosyldiacylglycerols, SQDGs). The main fatty acids in SQDGs were palmitic acid (C16:0), 33.3%; EPA (C20:5), 30.0%; arachidonic acid (C20:4), 12.7%; oleic acid (C18:1), 7.52%; and stearic acid (C18:0), 6.83%. The n-3/n-6 ratio was 1.9, whereas the authentic standard, spinach SQDG, did not contain n-3 fatty acids. Sulfoglycolipids inhibited the growth of human hepatocellular carcinoma cell line (HepG2). The IC50 was 126 μg/mL,which is lower than that of the spinach SQDG (255 μg/mL).
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