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Please use this identifier to cite or link to this item:
https://dyhuir.dyhu.edu.tw/ir/handle/987654321/232
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| Title: | 利用傳統變異法篩選L-色胺酸生產菌株之研究 |
| Authors: | 楊昭順;蘇遠志 |
| Keywords: | 傳統變異法;L 色胺酸;菌株 |
| Date: | 1997/11
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| Issue Date: | 2011-08-17T05:17:20Z
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| Abstract: | 本研究利用紫外線照射及EMS處理L-色胺酸生產菌株，進行誘導變異處理，得到具有2mg/mL TyrHx、4mg/mL SG及1mg/mL TrpHx類似化合物耐性之菌株 Brevibacterium divaricatum No.13
利用B. divaricatum No.13菌株以500ml Hinton 氏振盪培養瓶探討L-色胺酸生產之最適生產培養基成份為: Glucose 140g/L,(NH4)2SO4) 40g/L ,Yeast Extract 20g/L,KH2PO4) 1g/L, MgSO4．7H2O 0.4g/L, MnSO4．H2O 10mg/L, FeSO4．7H2O 10mg/L, Thiamine．Hcl 100μ g/L, d-Biotin 200 μ g/L, CaCO3 30g/L，裝液量30mL並以150rpm振盪培養84小時後，其醱酵液中累積5.14g/L之L-色胺酸。 |
| Relation: | 德育學報第十三期:091-098 |
| Appears in Collections: | [Journal of Deh Yu] Journal of Deh Yu 13
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